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attached and the like is available below.Trypanosoma brucei, a protozoan parasite, causes sleeping sickness, a fatal disease, in humans. Sleeping sickness is transmitted by the tsetse fly vector and occurs in the sub-Saharan tropics. The disease is a major public health problem in African countries, where hundreds of thousands of people are infected each year. The trypanosomes are transmitted by the bites of tsetse flies and invade human blood cells during the parasites’ journey from the fly’s gut through the fly’s salivary glands into the bloodstream. Following its invasion of the bloodstream, the parasite must be transported to the central nervous system, where it differentiates into bloodstream forms and replicates, ultimately destroying the human host. To ensure survival, the bloodstream trypanosomes must adapt to the environment of the bloodstream and must be capable of successfully navigating the circulatory system. The overall aim of this project is to define the contribution of the cytoskeleton to bloodstream-form trypanosome development and to dissect the molecular mechanism of blood cell invasion by the trypanosomes. Evidence in the literature indicates that actin and tubulin exist in both bloodstream and insect forms in their “cytoskeletal” state, but have different and complementary functions. However, the molecular details of how bloodstream-form trypanosomes are able to exploit the functions of actin and tubulin for invasion of the human host are not well-defined. The goal of this project is to examine the actin- and tubulin-based cytoskeletons and their role in bloodstream-form trypanosome development and in the invasion of human blood cells. For this purpose, two systems will be used: 1) The leukocyte host cell entry (LHE) assay will http://www.recetariodesirena.com/?p=13564
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